[Establishment of Wg3h-neo cell line with two genetic markers]

Plasmid PSV2-neo DNA containing neo gene (PSV2 neo) was successfully transferred into Wg3h cells, a HGPRT-Chinese hamster fibroblast cell line, by calcium phosphate DNA co-precipitating technique. Cells carrying two selectable markers (AGR and G418R) were thus obtained and passed for more than 40 generations, and designated as Wg3h-neo cell line. The existence of PSV2-neo sequences in the Wg3h-neo genome was confirmed by dot-blot hybridization with PSV2-neo DNA probe. Comparative studies showed that no significant difference in morphology, growth rate or genetic characteristics could be found between the Wg3h and Wg3h-neo cell lines.