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Mapping of the full profile of T cell allorecognition regions on HLA-DR2 beta subunit
1Department of Microbiology/Immunology, Baylor College of Medicine, Houston, Texas 77030.
Alloreactive human T cell lines were developed by repeated in vitro restimulation with alternate allogeneic cells of similar DR2 serotype, but differing at other HLA loci. These polyclonal T cells recognized DR specificities in common with all DR2 serotypes. The allorecognition profiles of DR2 beta by the T cell lines were mapped by consecutive uniform-sized overlapping peptides encompassing the entire extracellular and intracellular regions of the DR beta subunit. It was found that the T cell allorecognition sites are focused around the first and third polymorphic regions of the N-terminal domain, and interestingly, include conserved areas in the DR beta second domain as well as the intracellular segment beta 222-237. These findings have important implications for allorecognition. Comparison with the previous analysis of antibody-binding regions of DR2 beta revealed some similarities and differences in the antibody and T cell alloresponses to this histocompatibility antigen.
Alloreactive human T cell lines were developed by repeated in vitro restimulation with alternate allogeneic cells of similar DR2 serotype, but differing at other HLA loci. These polyclonal T cells recognized DR specificities in common with all DR2 serotypes. The allorecognition profiles of DR2 beta by the T cell lines were mapped by consecutive uniform-sized overlapping peptides encompassing the entire extracellular and intracellular regions of the DR beta subunit. It was found that the T cell allorecognition sites are focused around the first and third polymorphic regions of the N-terminal domain, and interestingly, include conserved areas in the DR beta second domain as well as the intracellular segment beta 222-237. These findings have important implications for allorecognition. Comparison with the previous analysis of antibody-binding regions of DR2 beta revealed some similarities and differences in the antibody and T cell alloresponses to this histocompatibility antigen.