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Related Experiment Videos

Processing of precursor proteins by plant mitochondria.

J Whelan1, P O'Mahony, M A Harmey

  • 1Department of Botany, University College Dublin, Belfield, Ireland.

Archives of Biochemistry and Biophysics
|June 1, 1990
PubMed
Summary

Plant mitochondria can process fungal precursor proteins, yielding mature forms. This cross-kingdom processing involves two protein components, one new and one related to Neurospora proteins.

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Area of Science:

  • Mitochondrial protein import and processing
  • Plant-fungal interactions
  • Biochemistry

Background:

  • Neurospora crassa precursor proteins require specific processing for mitochondrial import.
  • Plant mitochondria possess matrix processing activities.
  • Cross-kingdom protein processing is not well understood.

Purpose of the Study:

  • To investigate if plant mitochondria can process fungal precursor proteins.
  • To characterize the components responsible for this cross-kingdom processing activity.
  • To compare processing of different Neurospora proteins by plant mitochondria.

Main Methods:

  • In vitro processing assays using matrix extracts from Vicia faba and cauliflower mitochondria.
  • Incubation of Neurospora crassa precursor proteins (F1 beta subunit of F0F1 ATPase, subunit V of Rieske FeS complex) with plant mitochondrial extracts.

Related Experiment Videos

  • Analysis of processed proteins by molecular mass.
  • Immunological characterization using antibodies against Neurospora processing proteins.

    • Main Results:

    • Plant mitochondrial extracts correctly processed Neurospora precursor proteins to mature forms.
    • The processing activity involved two components: one antigenically related to Neurospora processing enhancing protein, and a second novel protease.
    • The F1 beta subunit was fully processed, while subunit V of the Rieske FeS complex was only partially processed.
    • Plant mitochondrial processing did not account for all observed processing during in vivo import.

    Conclusions:

    • Plant mitochondria exhibit cross-kingdom protein processing capabilities.
    • This processing involves both known and novel protein factors.
    • Plant mitochondrial processing differs from fungal systems and does not fully replicate in vivo import events.