Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An observational cross-sectional study of pharmaceutical waste disposal practices in Australian medical imaging departments: A comparison of community versus hospital practice.

Radiography (London, England : 1995)·2024

Same author

The influence of neonatal BCG vaccination on in vitro cytokine responses to Plasmodium falciparum.

BMC immunology·2024

Same author

Fetal fraction-based risk algorithm for non-invasive prenatal testing: screening for trisomies 13 and 18 and triploidy in women with low cell-free fetal DNA.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2018

Same author

The establishment of DOHaD working groups in Australia and New Zealand.

Journal of developmental origins of health and disease·2016

Same author

Extended right hemicolectomy and left hemicolectomy for colorectal cancers between the distal transverse and proximal descending colon.

Annals of the Royal College of Surgeons of England·2016

Same author

The use of infliximab in X-linked agammaglobulinaemia associated enteropathy.

Annals of the Royal College of Surgeons of England·2014

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

Transverse alternating-field electrophoresis.

¹Eleanor Roosevelt Institute for Cancer Research, Denver, CO.

Methods in Molecular Biology (Clifton, N.J.)

|March 17, 2011

Summary

Transverse alternating-field electrophoresis (TAFE) uses a vertical gel and unique electrode configuration for DNA separation. This pulsed-field technique offers distinct field angles for improved molecular analysis.

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Pulsed-field gel electrophoresis (PFGE) techniques are crucial for separating large DNA molecules.
Traditional PFGE methods often involve complex electrode arrangements.
A need exists for simplified yet effective PFGE systems.

Purpose of the Study:

To describe the apparatus and principles of Transverse Alternating-Field Electrophoresis (TAFE).
To highlight the unique vertical gel and electrode geometry of TAFE.
To explain the DNA migration patterns influenced by varying electric field angles.

Main Methods:

TAFE employs a vertical gel supported within an electrophoresis tank.
A simple electrode geometry with wires across the tank width is utilized.

More Related Videos

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Published on: March 31, 2019

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

Published on: May 7, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Electrophoretic Analysis of Replication Through Structure-Prone DNA Repeats Within the SV40-Based Human Episome

Published on: September 13, 2024

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Published on: March 31, 2019

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

Published on: May 7, 2017

Alternating electric fields (A and B) are applied to move DNA molecules.

Main Results:

DNA molecules are alternately moved downward and left, then downward and right.
The angle between the electric fields (A and B) varies along the gel's length.
The downward component of the electric field decreases with increasing distance from the wells.

Conclusions:

TAFE provides a simplified approach to pulsed-field gel electrophoresis.
The variable electric field angles in TAFE influence DNA migration.
This technique offers a novel method for analyzing large DNA fragments.