Related Experiment Video
Updated: Jun 3, 2026
05:49
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis
A P Monaco1, Z Lark, H Lehrach
1Human Genetics Laboratory, Imperial Cancer Research Fund, Institute of Molecu1ar Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Methods in Molecular Biology (Clifton, N.J.)
|March 17, 2011
Abstract:
Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries.
