Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility, suggesting a...
Type I Diabetes II: Pathophysiology01:26

Type I Diabetes II: Pathophysiology

Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...
Type II Diabetes II: Pathophysiology01:24

Type II Diabetes II: Pathophysiology

PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.
Diabetes Mellitus: Introduction01:26

Diabetes Mellitus: Introduction

Diabetes mellitus consists of chronic metabolic disorders characterized by persistent hyperglycemia. This elevated blood glucose results from defects in insulin secretion, impaired insulin action, or both. Insulin, produced by pancreatic β-cells, is essential for maintaining glucose homeostasis by facilitating cellular glucose uptake for energy or storage. Disruptions in insulin production or function lead to glucose accumulation in the bloodstream, causing the clinical features and long-term...
Diabetic Nephropathy01:28

Diabetic Nephropathy

Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preexisting diabetes and pregnancy: reflections on the joint guidelines of the Endocrine Society and European Society of Endocrinology.

Acta diabetologica·2026
Same author

Immune Cell Therapy Promises More Effective Cure for Medulloblastoma.

Journal of personalized medicine·2026
Same author

Impact of controlled type 2 diabetes on muscle-tendon mechanics.

Acta diabetologica·2026
Same author

Pregnancy after bariatric surgery: a point of view on unresolved questions.

Journal of endocrinological investigation·2026
Same author

Does the B7-H3 Immune Checkpoint Have High Potential as a Therapeutic Target?

Cells·2026
Same author

The Search for Predictive Biomarkers in Response to Immune Checkpoint Inhibitors and Associated Adverse Events.

Journal of personalized medicine·2025

Related Experiment Video

Updated: Jun 3, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Some views on proteomics in diabetes.

Annunziata Lapolla1, Simona Porcu, Pietro Traldi

  • 1Università di Padova, Dipartimento di Scienze Mediche e Chirurgiche, Padova, Italy. annunziata.lapolla@unipd.it

Clinical Chemistry and Laboratory Medicine
|March 18, 2011
PubMed
Summary

Mass spectrometry aids diabetes monitoring by analyzing protein glycation and urinary changes. This analytical tool offers a comprehensive view of disease progression and complications like nephropathy.

More Related Videos

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Related Experiment Videos

Last Updated: Jun 3, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Clinical Diagnostics

Background:

  • Diabetes mellitus is a complex metabolic disorder.
  • Protein glycation is a key pathological process in diabetes.
  • Diabetes-induced nephropathy is a significant long-term complication.

Purpose of the Study:

  • To review the application of mass spectrometry in diabetes research.
  • To highlight mass spectrometry's role in understanding glycation and its consequences.
  • To demonstrate mass spectrometry's utility in monitoring diabetes and its complications.

Main Methods:

  • Mass spectrometry (MS) coupled with liquid chromatography.
  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for glycation degree evaluation.
  • Proteomic approaches for identifying glycation sites and modifications.

Main Results:

  • Mass spectrometry effectively investigates protein modifications from glycation.
  • MALDI/MS quantifies glycation in plasma proteins.
  • Proteomics identifies glycation sites and urinary protein profile changes in diabetic nephropathy.

Conclusions:

  • Mass spectrometry is crucial for diabetes monitoring.
  • It provides insights into physiopathological changes during diabetes development.
  • This technology enhances understanding of diabetes-related complications like nephropathy.