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Updated: Jun 3, 2026

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
Mycobacterium bovis antigens for the differential diagnosis of vaccinated and infected cattle
Martin Vordermeier1, Stephen V Gordon, R Glyn Hewinson
1Veterinary Laboratories Agency - Weybridge, TB Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK. m.vordermeier@vla.defra.gsi.gov.uk
Abstract:
The urgency for new and improved cattle vaccines and diagnostic reagents for Bovine tuberculosis (TB) has made their development a research priority in Great Britain (GB). Significant progress has been made to develop specific antigens that allow the differentiation of BCG vaccinated and Mycobacterium bovis infected cattle (DIVA test). This has been greatly facilitated by the completion of the genome sequences of M. tuberculosis, M. bovis and BCG Pasteur and the subsequent application of comparative genome and transcriptome analysis to define DIVA antigens that complemented the prototype DIVA antigens ESAT-6 and CFP-10 by increasing their test sensitivity. Finally, we present an up-date of our current approaches in this area.

