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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

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Related Experiment Video

Updated: Jun 3, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Tight function zonula occludens-3 regulates cyclin D1-dependent cell proliferation.

Christopher T Capaldo1, Stefan Koch, Michael Kwon

  • 1Epithelial Pathobiology Research Unit, Department of Pathology, Emory University, Atlanta, GA 30322, USA.

Molecular Biology of the Cell
|March 18, 2011
PubMed
Summary

Epithelial tight junctions (TJs) regulate cell proliferation by sequestering cyclin D1 during mitosis. This interaction prevents premature cyclin D1 degradation, maintaining cell-cycle control and tissue homeostasis.

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Last Updated: Jun 3, 2026

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Published on: May 3, 2018

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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Epithelial tissue homeostasis relies on controlled cell proliferation.
  • Uncontrolled proliferation is a hallmark of cancer.
  • Epithelial tight junctions (TJs) are implicated in cell proliferation, but mechanisms are unclear.

Purpose of the Study:

  • Identify and characterize a novel plasma membrane pool of cyclin D1.
  • Elucidate the role of TJ proteins in regulating cyclin D1 and cell proliferation.

Main Methods:

  • Investigated cyclin D1 localization and function at the plasma membrane.
  • Utilized mutagenesis and siRNA to disrupt cyclin D1/ZO complexes.
  • Assessed cyclin D1 proteolysis and cell-cycle progression.

Main Results:

  • Identified a novel plasma membrane pool of cyclin D1 involved in cell-cycle regulation.
  • Discovered that ZO proteins sequester cyclin D1 at TJs during mitosis via a PDZ-binding motif.
  • Disruption of the cyclin D1/ZO complex led to increased cyclin D1 degradation and cell-cycle arrest.

Conclusions:

  • ZO family TJ proteins stabilize cyclin D1 during mitosis.
  • This stabilization is crucial for regulating epithelial cell proliferation.
  • TJ proteins play a significant role in maintaining epithelial homeostasis and preventing carcinogenesis.