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Related Experiment Videos

Peptidyl dipeptidase in rabbit brain microvessels.

P Brecher, A Tercyak, H Gavras

    Biochimica Et Biophysica Acta
    |October 12, 1978
    PubMed
    Summary

    Angiotensin-converting enzyme activity was found in rabbit brain microvessels. This suggests a physiological role for this enzyme in the brain's blood vessels.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cardiovascular Biology

    Background:

    • Angiotensin-converting enzyme (ACE) is a key enzyme in the renin-angiotensin system.
    • ACE plays a crucial role in regulating blood pressure and fluid balance.
    • Its presence and function in extrapulmonary vascular beds are not fully understood.

    Purpose of the Study:

    • To investigate the activity and localization of peptidyl dipeptidase (angiotensin-converting enzyme) in cerebral microvessels.
    • To determine if ACE has a physiological role in the brain's vasculature.

    Main Methods:

    • Studied enzymatic activity in isolated rabbit brain small blood vessel preparations (arterioles and capillaries).
    • Used hippuryl-histidyl-leucine and tritium-labeled angiotensin I as substrates for enzyme activity assays.
    • Assessed the effect of chloride ions and ACE inhibitors on enzymatic activity.

    Main Results:

    • Demonstrated significant peptidyl dipeptidase activity in rabbit cerebral microvessel homogenates.
    • Enzyme activity was dependent on chloride ions and inhibited by known ACE inhibitors.
    • Specific activity in microvessels was 20-fold higher than in whole brain homogenates and 60% of that in rat lung homogenates.

    Conclusions:

    • Peptidyl dipeptidase is present in the cerebral vasculature, likely localized to endothelial cells.
    • These findings support a physiological role for ACE in extrapulmonary vascular beds, including the brain.
    • The study highlights the importance of ACE in cerebral blood regulation.

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