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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 3, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Peptide aptamer microarrays: bridging the bio-detector interface.

Qifeng Song¹, Lukas Kurt Josef Stadler, Jianhe Peng

¹Section of Experimental Therapeutics, Wellcome Trust Brenner Building, St James's University Hospital, Beckett Street, Leeds, LS9 7TF.

Faraday Discussions

|March 18, 2011

Summary

This summary is machine-generated.

Researchers developed novel protein probes that are stable on surfaces, enabling multiplexed detection of disease biomarkers for personalized medicine. These non-antibody probes offer a promising advancement for clinical diagnostics and treatment response prediction.

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Last Updated: Jun 3, 2026

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Published on: January 7, 2019

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Published on: November 18, 2014

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

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Published on: October 6, 2022

Area of Science:

Biomarker Discovery
Molecular Diagnostics
Protein Engineering

Background:

Personalized medicine requires moving beyond single biomarkers to molecular signatures for diagnosis and treatment prediction.
Current diagnostic tests face limitations in multiplexed detection of various biomarkers (proteins, RNA, metabolites) due to antibody behavior in solution versus on surfaces.

Purpose of the Study:

To engineer novel, surface-stable protein probes for multiplexed biomarker detection.
To overcome the limitations of traditional antibodies in surface-based diagnostic assays.

Main Methods:

Engineered a non-antibody scaffold protein derived from human Stefin A, modifying its binding surfaces.
Developed designer probes with high specificity and antibody-like binding affinities.
Utilized these probes in various detection formats including microarrays, surface plasmon resonance, QCM, microcantilevers, and electrochemical assays.

Main Results:

The engineered protein probes demonstrated stability and high specificity on surfaces.
Successful multiplexed detection of labeled and label-free proteins from cell lysates was achieved.
Probes were effective in probing biology within intact cells.

Conclusions:

Optimized surface chemistry, robust designer probes, and sensitive detection technologies are key for clinical multiplexed biomarker detection.
This approach holds significant potential for advancing clinical diagnostics, particularly in areas like cancer detection where multiple biomarkers are relevant.