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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 3, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Peptide-tags for enhanced DNA microarray performance.

Holger Schulze1, Alan J Ross, Stuart W J Ember

  • 1Division of Pathway Medicine, College of Medicine and Veterinary Medicine, The University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh, EH16 4SB, Scotland. holger.schulze@ed.ac.uk

Faraday Discussions
|March 18, 2011
PubMed
Summary
This summary is machine-generated.

Histidine-tagged DNA probes significantly enhance hybridization efficiency and sensitivity in DNA microarrays. This breakthrough offers improved detection limits for research and clinical diagnostic applications.

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Peptide-based Identification of Functional Motifs and their Binding Partners
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Peptide-based Identification of Functional Motifs and their Binding Partners

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

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Last Updated: Jun 3, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Surface Chemistry

Background:

  • DNA microarrays are essential for gene expression analysis and diagnostics.
  • Clinical applications require high sensitivity and rapid results.
  • Probe immobilization impacts microarray hybridization efficiency.

Purpose of the Study:

  • To optimize DNA probe immobilization for enhanced microarray performance.
  • To investigate the role of oligonucleotide modifications in hybridization efficiency.
  • To explore the binding mechanisms of histidine-tags on functionalized surfaces.

Main Methods:

  • Tested various oligonucleotide modifications on epoxy silane functionalized glass slides.
  • Utilized histidine-tagged oligonucleotides for probe immobilization.
  • Investigated different peptide-tags to understand binding mechanisms.

Main Results:

  • Histidine-tagged oligonucleotides demonstrated the highest bound probe amounts and hybridization efficiencies.
  • Detection limits were improved by up to two orders of magnitude compared to standard modifications.
  • Terminal amino groups influenced covalent binding, while aromatic residues enhanced hybridization.

Conclusions:

  • Histidine-tagging is a superior method for DNA probe immobilization on glass slides.
  • This technique significantly improves DNA microarray sensitivity and efficiency.
  • Understanding tag-surface interactions can further optimize microarray design for clinical diagnostics.