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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Development of single-stranded DNA aptamers for specific Bisphenol a detection.

Minjoung Jo1, Ji-Young Ahn, Joohyung Lee

  • 1Department of Biomedical Engineering, Dongguk University , Seoul, Korea.

Oligonucleotides
|March 19, 2011
PubMed
Summary

Researchers developed specific DNA aptamers to detect Bisphenol A (BPA), an environmental hormone. This aptamer-based biochip offers a novel method for high-throughput detection of BPA in water samples.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Environmental Science

Background:

  • High-affinity and specific reagents are essential for detecting small molecules like toxicants.
  • Aptamers, short single-stranded DNA (ssDNA) oligonucleotides, offer high affinity for target molecule recognition.

Purpose of the Study:

  • To select and characterize ssDNA aptamers that specifically bind to Bisphenol A (BPA).
  • To develop a novel aptamer-based detection method for BPA in environmental samples.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process was employed to isolate BPA-binding aptamers.
  • Selected aptamers were tested for specificity against structurally similar compounds.
  • An aptamer-based sol-gel biochip was constructed for BPA detection.

Main Results:

  • High-affinity ssDNA aptamers recognizing BPA were successfully selected from a large library.
  • The selected aptamers demonstrated high specificity for BPA, distinguishing it from similar molecules like Bisphenol B.
  • A functional aptamer-based sol-gel biochip was developed, enabling BPA detection in water.

Conclusions:

  • Novel ssDNA aptamers with high affinity and specificity for BPA have been developed.
  • The aptamer-based sol-gel biochip provides a sensitive platform for BPA detection.
  • This approach holds potential for the universal, high-specificity detection of various small molecules.