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Microchip extraction of catecholamines using a boronic acid functional affinity monolith.

Cafer Cakal¹, Jerome P Ferrance, James P Landers

¹Hacettepe University, The Inst. for Grad. Studies in Sci. and Eng., Bioeng. Subdivision, Ankara, Turkey.

Analytica Chimica Acta

|March 19, 2011

Summary

This study introduces a novel microchip for extracting catecholamines using boronic acid affinity. The developed solid phase extraction microchip offers over 100-fold enrichment for cis-diol compounds.

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Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Area of Science:

Analytical Chemistry
Materials Science
Biochemistry

Background:

Catecholamines are vital biomarkers, but their low concentrations necessitate efficient extraction methods.
Solid phase extraction (SPE) is a common technique, but microchip formats offer advantages in miniaturization and efficiency.
Boronic acid functional materials show affinity for cis-diol containing compounds like catecholamines.

Purpose of the Study:

To develop a novel SPE microchip utilizing a boronic acid functional monolithic disc for catecholamine extraction.
To investigate the adsorption and desorption characteristics of the developed material for catecholamines.
To demonstrate the efficiency of the microchip for concentrating catecholamines using electrokinetic flow and laser-induced native fluorescence detection.

Main Methods:

Synthesis of vinyl phenylboronic acid-ethylene glycol dimethacrylate co-polymer monoliths.
Fabrication of monolithic discs within borofloat glass microfluidic chips via in situ UV polymerization.
Batch adsorption and desorption studies to optimize catecholamine recovery.
Integration of electrokinetic flow for adsorption and elution, coupled with laser-induced native fluorescence (LINF) detection.

Main Results:

Achieved >90% desorption yields for catecholamines at pH 3 and below.
Demonstrated >100-fold concentration enrichment of catecholamines using the SPE microchip.
Successfully extracted and detected catecholamines via electrokinetic elution and LINF.

Conclusions:

The developed SPE microchip with a boronic acid functional monolithic disc is effective for catecholamine extraction.
The microchip offers a highly sensitive and efficient platform for analyzing cis-diol containing compounds.
This technology holds promise for miniaturized analytical systems in various biomedical applications.