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Dual-fluorophore ratiometric pH nanosensor with tuneable pKa and extended dynamic range
Veeren M Chauhan1, Gary R Burnett, Jonathan W Aylott
1Laboratory of Biophysics and Surface Analysis, School of Pharmacy, Boots Sciences Building, University Park, Nottingham, UK NG7 2RD.
Abstract:
Ratiometric pH nanosensors with tuneable pK(a) were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green(®) dextran (OG-D)) and a reference fluorophore (5-(and-6)-carboxytetramethylrhodamine dextran (TAMRA-D)), in a biocompatible polymer matrix. Dual-fluorophore pH nanosensors permit the measurement of an extended dynamic range, from pH 4.0 to 7.5.
