Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuberculous tenosynovitis and bursitis: imaging findings in 21 cases.

Radiology·1996
Same author

Recombinant mitotoxin basic fibroblast growth factor-saporin reduces venous anastomotic intimal hyperplasia in the arteriovenous graft.

Circulation·1996
Same author

Measurement of urinary estrogen metabolites using a monoclonal enzyme-linked immunoassay kit: assay performance and feasibility for epidemiological studies.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·1996
Same author

Differences in cholinergic responses from outer hair cells of rat and guinea pig.

Hearing research·1996
Same author

Pharmacokinetics of retinoids in women after meal consumption or vitamin A supplementation.

Journal of clinical pharmacology·1996
Same author

Determination of the amino acid residue involved in [3H]beta-funaltrexamine covalent binding in the cloned rat mu-opioid receptor.

The Journal of biological chemistry·1996

Related Experiment Video

Updated: Jun 3, 2026

Calcium Phosphate Transfection of Primary Hippocampal Neurons
08:22

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Published on: November 12, 2013

Calcium phosphate mediated gene transfer into established cell lines.

H Okayama1, C Chen

  • 1Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD, Osaka, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2011
PubMed
Summary

DNA transfection is a vital molecular genetics technique. It enables the study of eukaryotic gene function and isolation of genes based on cellular expression.

More Related Videos

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Related Experiment Videos

Last Updated: Jun 3, 2026

Calcium Phosphate Transfection of Primary Hippocampal Neurons
08:22

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Published on: November 12, 2013

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Area of Science:

  • Molecular genetics
  • Gene expression analysis
  • Eukaryotic gene studies

Background:

  • DNA transfection is a cornerstone technique in molecular genetics.
  • It facilitates the detailed analysis of complex eukaryotic genes and their components.
  • This method is crucial for understanding gene function and regulation.

Purpose of the Study:

  • To highlight the significance of DNA transfection in molecular genetics research.
  • To underscore its role in dissecting eukaryotic gene structure and function.
  • To emphasize its utility in gene isolation via cultured cell expression.

Main Methods:

  • Gene dissection techniques
  • Functional characterization of gene components
  • Gene isolation through expression in cultured cells

Main Results:

  • Enabled dissection of complex eukaryotic genes.
  • Facilitated characterization of gene component functions.
  • Allowed isolation of specific genes based on cellular expression.

Conclusions:

  • DNA transfection is indispensable for advancing molecular genetics.
  • The technique provides powerful tools for gene function elucidation.
  • It is essential for isolating and studying genes in various biological contexts.