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Updated: Jun 3, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Published on: November 23, 2015

Patterned resonance plasmonic microarrays for high-performance SPR imaging.

Abdennour Abbas1, Matthew J Linman, Quan Cheng

  • 1Department of Chemistry, University of California, Riverside, California 92521, USA.

Analytical Chemistry
|March 23, 2011
PubMed
Summary
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This study introduces a new optical platform for surface plasmon resonance (SPR) imaging, achieving background-free images and high signal-to-noise ratios for advanced biosensing applications.

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Materials Science

Background:

  • Surface Plasmon Resonance (SPR) imaging is a powerful label-free technique for detecting molecular interactions.
  • Existing SPR imaging (SPRi) platforms often suffer from background noise and limited resolution.
  • Advancements are needed to improve sensitivity and enable real-time analysis of biological systems.

Purpose of the Study:

  • To develop a novel optical platform for SPR generation and confinement within a 3D microwell geometry.
  • To achieve background-free SPR images with enhanced signal-to-noise ratio (S/N) and thickness resolution.
  • To demonstrate the platform's efficacy in real-time analysis of lipid membrane arrays and microfluidic circuits.

Main Methods:

  • Utilized a unique three-dimensional microwell geometry for SPR generation and confinement.

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Last Updated: Jun 3, 2026

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  • Engineered an array with high signal-to-noise ratio (>80) and subnanometric thickness resolution.
  • Achieved an angular sensitivity of 1°/0.01 RIU and an improved signal-to-background ratio (S/B) of 20.

    • Main Results:

    • Demonstrated background resonance-free SPR images.
    • Achieved subnanometric thickness resolution (0.24 nm) for probing-supported lipid membrane arrays in real time.
    • Enabled imaging analysis of microfluidic circuits with high resolution (7 microm pixel separation).
    • Reported a signal-to-background ratio (S/B) of 20, an order of magnitude higher than existing literature.

    Conclusions:

    • The novel optical platform offers superior image quality and a unique chip geometry for SPRi detection.
    • This technology opens new avenues for high-throughput array screening and advanced biomicrofluidics.
    • The platform's high sensitivity and resolution are suitable for real-time monitoring of biological processes.