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Bilirubin peak can be mistaken as Hb Bart's or Hb H on High-performance liquid chromatography

Rakhee Kar1, Chaggan Bihari Sharma

  • 1Department of Clinical Haematology, Institute of Liver and Biliary Sciences, New Delhi, India. drrakheekar@gmail.com

Hemoglobin
|March 23, 2011
PubMed

High-performance liquid chromatography (HPLC) has largely replaced electrophoresis for the identification of hemoglobin (Hb) variants. However, one needs to be vigilant to pick up undue interference such as the presence of a bilirubin peak, which can be mistaken for either Hb Bart's or Hb H on Hb HPLC. Correlation with the clinical context and biochemical parameters, coupled with repeating the run after washing the sample, can resolve this problem. Herein, we report one such case of an acute on chronic liver failure patient who was found to have heterozygous β-thalassemia (β-thal) on Hb HPLC with an incidental tall unknown peak that was identified as bilirubin.

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