Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

Nucleoid

Nucleoid

The nucleoid represents a structurally and functionally distinct region within prokaryotic cells, where the cell's DNA and associated proteins are housed. Unlike eukaryotic cells, prokaryotes lack a membrane-bound nucleus, and the nucleoid facilitates the organization and accessibility of the genetic material within this constraint. The DNA in most bacteria and archaea exists as a single, circular, double-stranded molecule that is highly compacted through supercoiling and interactions with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Monomers containing 2'-o-alkoxymethyl groups as synthons for the synthesis of oligoribonucleotides by the phosphotriester method].

Bioorganicheskaia khimiia·2012

Same author

[Application of BODIPY-trimethylmelamine conjugate for DNA cross-linking in vitro].

Bioorganicheskaia khimiia·2011

Same author

[Methoxymethyl and (p-nitrobenzyloxy)methyl groups in the synthesis of oligoribonucleotides by the phosphotriester method].

Bioorganicheskaia khimiia·2011

Same author

[DNA mimics on the base of pyrrolidine and hydroxyproline].

Bioorganicheskaia khimiia·2011

Same author

[N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligonucleotides].

Bioorganicheskaia khimiia·2010

Same author

[Cross-linked nucleic acids: formation, structure, and biological function].

Bioorganicheskaia khimiia·2010

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Cross-linked nucleic acids: isolation, structure, and biological role.

V A Efimov¹, S V Fedyunin

¹Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. eva@mx.ibch.ru

Biochemistry. Biokhimiia

|March 23, 2011

Summary

This summary is machine-generated.

This review covers nucleic acid cross-linking agents, discussing their reactivity, binding sites, and detection methods. It also explores cellular responses and therapeutic applications of these cross-linking reagents.

More Related Videos

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Related Experiment Videos

Last Updated: Jun 3, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Area of Science:

Molecular Biology
Biochemistry
Medicinal Chemistry

Background:

Nucleic acids are fundamental biomolecules.
Covalent cross-linking agents modify nucleic acids.
Understanding these modifications is crucial for biological and medical applications.

Purpose of the Study:

To review literature on reagents that induce covalent cross-links in nucleic acids.
To discuss the reactivity and binding preferences of these agents.
To explore the biological consequences and applications of nucleic acid cross-linking.

Main Methods:

Literature review of existing studies.
Analysis of chemical properties of cross-linking agents.
Discussion of methods for identifying cross-link locations.
Examination of cellular responses to nucleic acid cross-linking.

Main Results:

Identified main types of nucleic acid cross-linking reagents.
Discussed agent reactivity and preferred nucleic acid binding sites.
Reviewed methods for determining cross-link locations within duplexes.
Detailed cellular responses including replication/transcription blocking, DNA repair, and apoptosis.

Conclusions:

Cross-linking agents have diverse reactivity and binding specificities.
Methods exist to locate cross-links in nucleic acid duplexes.
Nucleic acid cross-linking triggers significant cellular responses.
These reagents hold potential as therapeutic agents in molecular biology.