Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolomics profiling reveals changes in peptidoglycan and redox metabolism between ancient and modern <i>Mycobacterium tuberculosis</i>.

Microbiology spectrum·2026
Same author

A noninvasive BCG skin challenge model for assessing tuberculosis vaccine efficacy.

PLoS biology·2024
Same author

Piloting delivery of PfSPZ vaccines for malaria through a cryogenic vaccine cold chain to travel and military medicine clinics.

Journal of travel medicine·2024
Same author

An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses.

PLoS pathogens·2023
Same author

Practical considerations for a TB controlled human infection model (TB-CHIM); the case for TB-CHIM in Africa, a systematic review of the literature and report of 2 workshop discussions in UK and Malawi.

Wellcome open research·2023
Same author

Galleria mellonella-intracellular bacteria pathogen infection models: the ins and outs.

FEMS microbiology reviews·2023

Related Experiment Video

Updated: Jun 3, 2026

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
07:28

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Published on: November 24, 2017

A modified agar pad method for mycobacterial live-cell imaging.

Graham Joyce1, Brian D Robertson, Kerstin J Williams

  • 1Centre for Molecular Microbiology and Infection, Imperial College, London, SW7 2AZ, UK. Kerstin.Williams@imperial.ac.uk.

BMC Research Notes
|March 23, 2011
PubMed
Summary
This summary is machine-generated.

A modified agar pad method enables long-term live imaging of bacteria like Mycobacterium. This technique allows for environmental manipulation during experiments, overcoming limitations of previous methods for prokaryotic cell studies.

More Related Videos

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
07:31

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Published on: July 28, 2011

Related Experiment Videos

Last Updated: Jun 3, 2026

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
07:28

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Published on: November 24, 2017

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
07:31

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Published on: July 28, 2011

Area of Science:

  • Microbiology
  • Cell Biology
  • Microscopy

Background:

  • Traditional prokaryotic live-cell imaging methods include agar pads and micro-channels.
  • Agar pad methods, while simple, restrict environmental manipulation and long-term studies due to sealed conditions.

Purpose of the Study:

  • To develop an improved agar pad method for enhanced prokaryotic live-cell imaging.
  • To overcome limitations of existing agar pad techniques for extended observation periods.

Main Methods:

  • A modified agar pad technique was developed, reversing the typical arrangement.
  • Cells were sandwiched between a coverslip (bottom) and agar pad (top).
  • Imaging was performed from below using an inverted microscope.

Main Results:

  • The modified method successfully produced time-lapse images and movies of cell growth.
  • Studied organisms included Mycobacterium smegmatis and Mycobacterium bovis BCG.
  • This approach overcomes limitations of previous agar pad methods.

Conclusions:

  • The revised agar pad method facilitates long-term live cell imaging studies.
  • This technique allows for media modification during experiments.
  • It provides an improved approach for studying slow-growing bacteria.