Related Experiment Video
Updated: Jun 3, 2026

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
Published on: November 8, 2024
Separation, identification and quantification of tetrodotoxin and its analogs by LC-MS without calibration of
Xiao-Wu Chen1, Hong-Xia Liu, Yi-Bao Jin
1Key Laboratory of Chemical Biology of Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518057, PR China.
Abstract:
We report here a simple and rapid method of separation, identification and quantification of tetrodotoxin (TTX) and its analogs in partially purified extract by LC-MS. Except for the main component calibration of individual components was not necessary. TTX and four of its analogs in the puffer fish extract were identified and quantified. The limits of detection and quantification, the linear range and accuracy of the protocol were validated.
More Related Videos
09:36Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
09:34Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Published on: September 20, 2016
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry