[Cloning of alpha-hemolysin gene from Staphylococcus aureus strain 015]

D K Meshveliani, R Sh Adamiia, R P Vashakidze

Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia

|March 1, 1990

Abstract:

The genomic library of Staphylococcus aureus O15 has been constructed on the EMBL-3 vector. The synthetic oligonucleotide probes to N- and C-end regions of alpha-hemolysin permitted identification of the recombinant bacteriophage clone RS-1 containing a gene for this protein. The restriction map of the cloned fragment has been constructed for restriction endonucleases SalGI, EcoRV, PstI, PvuII. Expression of the alpha-hemolysin gene in phagolysate of the recombinant clone RS-1 (1000 units per ml) has been demonstrated.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

[Identification of the gene whose mutation leads to the appearance of recessive lethal germlings in Arabidopsis thaliana].

Ontogenez·2005

[Identification of the gene whose mutation leads to hypocotyl tropism in Arabidopsis thaliana].

Genetika·2005

[Identification of the gene whose mutation leads to the morphological changes in the hypocotyl of Arabidopsis thaliana].

Genetika·2005

See all related articles

Related Experiment Videos

[Cloning of alpha-hemolysin gene from Staphylococcus aureus strain 015]

Related Concept Videos

[New Virulent Bacteriophages Active against Multiresistant Pseudomonas aeruginosa Strains].

[Identification of Arabidopsis thaliana genes whose expression determines changes in the shoot structure].

[New vector system for induction of gene expression in dicotyledonous plants].

[Identification of the gene whose mutation leads to the appearance of recessive lethal germlings in Arabidopsis thaliana].

[Identification of the gene whose mutation leads to hypocotyl tropism in Arabidopsis thaliana].

[Identification of the gene whose mutation leads to the morphological changes in the hypocotyl of Arabidopsis thaliana].

THE STRUCTURE OF THE INTESTINAL MICROBIOTA AND THE FREQUENCY OF DETECTION OF PATHOGENICITY GENES (STXI, STX2, BFP) IN ESCHERICHIA COLI WITH NORMAL ENZYMATIC ACTIVITY ISOLATED FROM CHILDREN DURING THE FIRST YEAR OF LIFE.

DISTRIBUTION OF RNA-CONTAINING BEE VIRUSES IN HONEY BEE (APIS MELLIFERA) IN SEVERAL REGIONS OF RUSSIA.

MOLECULAR EPIDEMIOLOGY OF TREPONEMA PALLIDUM IN BORDER REGION OF RUSSIAN FEDERATION (TUVA REPUBLIC).

PENELOPE-LIKE RETROTRANSPOSONS IN THE GENOME OF ASIAN BLOOD FLUKE SCHISTOSOMA JAPONICUM (TREMATODA: SCHISTOSOMATIDAE).

MOLECULAR-GENETIC PROPERTIES OF VIBRIO CHOLERAE EL TOR STRAINS CIRCULATING IN AFRICA.

MOLECULAR TYPING OF SHIGELLA STRAINS.

Plasma ALKBH5 depletion during severe intermittent hypoxia and its attenuation by galectin-3 inhibition: an experimental rat study.

Are the days gone when age mattered? A national-level comparison of frailty versus age in predicting postoperative outcomes.

Localizing the course of the radial nerve based on anatomical landmarks: A cadaveric study.

Echoes of Evolution in Holocentridae: Harmony Between Phylogeny, Morphology and Acoustics.

Effects of laparoscopic distal gastrectomy with Roux-en-Y anastomosis on postoperative gastrointestinal symptom recovery and bile reflux incidence in patients with gastric cancer.

Morphological and Morphometric Analysis of the Motor Branches of the Tibial Nerve Above the Soleus Arch: A Cadaveric Descriptive Study.

Related Experiment Videos

[Cloning of alpha-hemolysin gene from Staphylococcus aureus strain 015]

Abstract:

Related Concept Videos

Related Articles