Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Apoptosis

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size reduction of the tissue.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Factors that delay discovery in cases of death at home in Japan.

Legal medicine (Tokyo, Japan)·2026

Same author

Complementing Western Bioethics: Nishida Kitarō's Relational Ontology and the Future of Brain Organoid Research.

AJOB neuroscience·2025

Same author

Detection of myosin light chain 1 from a forensic biological material to identify human striated muscle.

Forensic science international·2025

Same author

Establishment and characterization of a novel cell line ICH-BCPALL-3 from B cell precursor acute lymphoblastic leukemia with TCF3::HLF.

Human cell·2025

Same author

Risk factors for aggravated COVID-19 despite medical care after admission among Japanese patients: A Japanese association for infectious diseases COVID registry study.

PloS one·2025

Same author

Respect Without Romanticizing: Cultural Values, Parental Reasons, and Unproven Pediatric Treatments in East Asia.

The American journal of bioethics : AJOB·2025

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Hydrogen sulfide causes apoptosis in human pulp stem cells.

Chie Kobayashi¹, Ken Yaegaki, Bogdan Calenic

¹Section of Periodontology, Department of Hard Tissue Engineering, Tokyo Medical and Dental University, Tokyo, Japan.

Journal of Endodontics

|March 23, 2011

Summary

Hydrogen sulfide (H2S) induces apoptosis in human pulp stem cells (HPSCs) by activating the mitochondrial pathway. This finding suggests H2S may contribute to pulpitis pathogenesis by reducing HPSC viability.

More Related Videos

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Related Experiment Videos

Last Updated: Jun 3, 2026

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Area of Science:

Dental Research
Stem Cell Biology
Molecular Pathogenesis

Background:

Dental caries can lead to pulpal inflammation, but pulpitis pathogenesis is not fully understood.
The role of human pulp stem cells (HPSCs) in pulpitis is unclear.
Bacterial hydrogen sulfide (H2S) may induce apoptosis in some tissues.

Purpose of the Study:

To investigate if H2S induces apoptosis in HPSCs.
To elucidate the signaling pathway involved in H2S-induced apoptosis in HPSCs.

Main Methods:

Human dental pulp stem cells were treated with H2S.
Apoptosis was detected using flow cytometry.
Mitochondrial pathway activation, caspase activity, and cytochrome C release were assessed.

Main Results:

H2S significantly increased HPSC apoptosis from 1.6% to 16.3%.
Mitochondrial membrane depolarization and release of cytochrome C increased significantly.
Caspase-9 and caspase-3 activation was observed, but caspase-8 was not.

Conclusions:

H2S induces apoptosis in HPSCs via the mitochondrial pathway.
H2S may contribute to pulpitis by causing HPSC loss of viability.