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Related Concept Videos

Microbes and Methanogenesis01:26

Microbes and Methanogenesis

Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...

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Related Experiment Video

Updated: Jun 3, 2026

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
04:32

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Published on: October 14, 2021

Methanotroph outer membrane preparation.

Odd A Karlsen1, Frode S Berven, Harald B Jensen

  • 1Department of Molecular Biology, University of Bergen, Bergen, Norway.

Methods in Enzymology
|March 23, 2011
PubMed
Summary
This summary is machine-generated.

Researchers isolated outer membrane proteins from Methylococcus capsulatus (Bath) bacteria. This study details methods for fractionating these proteins, aiding in understanding bacterial outer membrane composition.

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Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
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Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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Last Updated: Jun 3, 2026

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
04:32

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07:31

Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient

Published on: September 6, 2024

Area of Science:

  • Microbiology
  • Bacterial Cell Biology
  • Proteomics

Background:

  • Gram-negative bacteria possess a unique two-layered membrane structure: an inner and an outer membrane.
  • Methanotrophs, a class of bacteria, are exclusively Gram-negative, necessitating a deeper understanding of their membrane composition.
  • Methylococcus capsulatus (Bath) serves as a model organism for studying bacterial outer membrane structure and function.

Purpose of the Study:

  • To develop and present protocols for the isolation and fractionation of outer membrane proteins from Methylococcus capsulatus (Bath).
  • To identify proteins located in the outer leaflet of the bacterial outer membrane.
  • To contribute to the understanding of the protein and lipid composition of methanotroph outer membranes.

Main Methods:

  • Isolation of the outer membrane from purified cell envelopes using selective detergent solubilization (Triton X-100) of the inner membrane.
  • Fractionation of outer membrane proteins into integral/tightly associated and peripheral/loosely associated components.
  • Identification of outer leaflet proteins via whole-cell biotin surface labeling.

Main Results:

  • Successfully isolated and fractionated outer membrane proteins from Methylococcus capsulatus (Bath).
  • Developed a reliable method for distinguishing between different protein association types within the outer membrane.
  • Identified specific proteins present on the outer surface of the bacterial outer membrane using biotinylation techniques.

Conclusions:

  • The presented protocols enable detailed analysis of bacterial outer membrane protein composition.
  • This methodology is crucial for understanding the specific roles of outer membrane proteins in methanotrophs.
  • The findings provide a foundation for future studies on the structure-function relationships of bacterial outer membranes.