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Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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Isolation of methanobactin from the spent media of methane-oxidizing bacteria
Nathan L Bandow1, Warren H Gallagher, Lee Behling
1Department of Biochemistry, Iowa State University, Ames, Iowa, USA.
Methods in Enzymology
|March 23, 2011
Abstract:
Chalkophores are low molecular mass modified peptides involved in copper acquisition in methane-oxidizing bacteria (MOB). A screening method for the detection of this copper-binding molecule is presented in Chapter 16. Here we describe methods to (1) maximize expression and secretion of chalkophores, (2) concentrate chalkophores from the spent media of MOB, and (3) purify chalkophores.
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