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Development of andrographolide molecularly imprinted polymer for solid-phase extraction.

Xiaoying Yin¹, Qingshan Liu, Yifan Jiang

¹College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Wanli District, Nanchang, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|March 23, 2011

Summary

A novel molecularly imprinted polymer (MIP) solid-phase extraction (SPE) method selectively isolates andrographolide and dehydroandrographolide from Andrographis paniculata. This technique offers enhanced capacity and accuracy for sample pretreatment.

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Area of Science:

Analytical Chemistry
Polymer Science
Pharmacognosy

Background:

Andrographis paniculata (APN) is a medicinal herb containing bioactive compounds.
Selective extraction of active compounds like andrographolide is crucial for quality control and analysis.
Existing methods may lack the required selectivity and efficiency for complex herbal matrices.

Purpose of the Study:

To develop a selective solid-phase extraction (SPE) method using molecularly imprinted polymers (MIPs).
To evaluate the efficiency of MIP-SPE for the extraction of andrographolide and dehydroandrographolide from APN.
To compare the performance of MIP-SPE with conventional SPE methods.

Main Methods:

Preparation of MIPs using andrographolide as a template molecule via precipitation polymerization.
Characterization of MIP structure and measurement of static adsorption capacity using the Scatchard equation.
Application of MIP-SPE for sample pretreatment of Andrographis paniculata extracts and comparison with C(18)-SPE and non-imprinted polymer (NIP) SPE.

Main Results:

The MIP-SPE column demonstrated high selectivity and affinity for andrographolide and dehydroandrographolide.
MIP-SPE column capacity was significantly higher (11.9±0.6 μmol/g and 12.1±0.5 μmol/g) compared to C(18)-SPE and NIP-SPE (2-3 times higher).
The method achieved satisfactory precision and accuracy with high recoveries (96.4-103.8% for andrographolide, 96.0-104.2% for dehydroandrographolide) and low RSDs.

Conclusions:

Molecularly imprinted polymer solid-phase extraction (MIP-SPE) is a highly selective and effective technique for andrographolide and dehydroandrographolide.
The developed MIP-SPE method offers a superior alternative for rapid and efficient sample pretreatment of herbal medicines.
This approach holds significant potential for the analysis and quality control of Andrographis paniculata.