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Integrated polymer-based Mach-Zehnder interferometer label-free streptavidin biosensor compatible with injection

R Bruck¹, E Melnik¹, P Muellner¹

¹AIT Austrian Institute of Technology GmbH, Health & Environment, Nano Systems, Donau-City-Strasse 1, 1220 Vienna, Austria.

Biosensors & Bioelectronics

|March 23, 2011

Summary

This summary is machine-generated.

We developed a polymer Mach-Zehnder interferometer biosensor for label-free detection of biotin-streptavidin binding. This cost-effective optical sensor can detect low concentrations of streptavidin.

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Area of Science:

Biomedical Engineering
Materials Science
Optoelectronics

Background:

Mach-Zehnder interferometers are sensitive optical devices.
Polymer materials offer advantages for cost-effective sensor fabrication.
Label-free biosensing eliminates the need for secondary detection molecules.

Purpose of the Study:

To develop a polymer-based Mach-Zehnder interferometer biosensor.
To demonstrate label-free, real-time detection of biotin-streptavidin binding.
To evaluate the sensor's sensitivity and potential for mass production.

Main Methods:

Fabrication of a high index contrast polymer waveguide.
Functionalization of the waveguide surface with biotin.
Utilizing a fluidic system for introducing streptavidin solutions.
Monitoring binding events via changes in the interferometer's output signal at 1310 nm.

Main Results:

Successful label-free detection of biotin-streptavidin interactions.
Achieved a limit of detection for streptavidin as low as 0.1 μg/ml.
Demonstrated the feasibility of using polymer materials for optical biosensing despite lower surface sensitivity compared to inorganic systems.

Conclusions:

The polymer Mach-Zehnder interferometer biosensor enables sensitive, label-free detection of biomolecular binding.
The sensor's compatibility with mass production techniques like injection molding makes it a cost-effective alternative to inorganic sensors.
This technology holds promise for various diagnostic and research applications.