Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

Special Staining Techniques

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...

Simple Staining Technique

Simple Staining Technique

OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Implementing the collaborative chronic care model for mental health: a nonrandomized clinical trial.

Implementation science communications·2026

Same author

Duration of clinically meaningful improvement in pain as a patient-centered endpoint in acute pain trials.

Pain·2026

Same author

Provider and leadership attitudes toward telemental health at sites with high levels of video, phone, and in-person care: A qualitative analysis.

Psychological services·2026

Same author

Complication outcomes in retroauricular interpolated flaps after Mohs surgery: A single-center retrospective study.

Journal of the American Academy of Dermatology·2026

Same author

Impact of Wetting-Drying Cycles on Iron Oxide Transformations and Contaminant Mobilization in a Replica Legacy Radioactive Waste Trench.

Environmental science & technology·2026

Same author

Response to Xu et al., "Letter to Editor 'Mohs micrographic surgery reduces the risk of reconstruction with positive margins, repeat surgeries, and time to definitive reconstruction compared to conventional excision of hand and foot melanomas: A retrospective cohort study'".

Journal of the American Academy of Dermatology·2026

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Establishment of a Clinic-based Biorepository

Establishment of a Clinic-based Biorepository

Published on: May 29, 2017

Special stains in Mohs surgery.

Christopher J Miller¹, Joseph F Sobanko, Xiaodong Zhu

¹Department of Dermatology, Perelman Center for Advanced Medicine, University of Pennsylvania, 3400 Civic Center Boulevard, Suite 1-330S, Philadelphia, PA 19104, USA. christopher.miller@uphs.upenn.edu

Dermatologic Clinics

|March 23, 2011

Summary

Immunostaining enhances Mohs surgery by highlighting tumor cells, improving accuracy in distinguishing cancer from normal tissue. This technique is crucial for achieving high cure rates in dermatologic surgery.

More Related Videos

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Published on: August 11, 2023

Related Experiment Videos

Last Updated: Jun 3, 2026

Establishment of a Clinic-based Biorepository

Establishment of a Clinic-based Biorepository

Published on: May 29, 2017

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Published on: August 11, 2023

Area of Science:

Dermatologic Surgery
Oncology
Histopathology

Background:

Mohs surgery relies on precise microscopic interpretation of frozen sections.
Distinguishing tumor from normal tissue is critical for successful Mohs surgery outcomes.
Accurate slide interpretation is essential for high cure rates in Mohs surgery.

Purpose of the Study:

To review the practical applications of common immunostains in dermatologic surgery.
To highlight how immunostaining aids in differentiating neoplastic cells during Mohs surgery.
To discuss specific stains used for various skin neoplasms.

Main Methods:

Focus on practical aspects of commonly used immunostains in dermatologic surgery.
Discusses MART-1 for melanocytic neoplasms.
Discusses cytokeratin stains for keratinocytic neoplasms and CD34 for dermatofibrosarcoma protuberans.

Main Results:

Immunostaining effectively highlights tumor cells using chromogens visible under light microscopy.
This allows Mohs surgeons to reliably distinguish tumor cells from surrounding normal tissue.
Specific stains (MART-1, cytokeratins, CD34) are valuable for identifying different neoplasm types.

Conclusions:

Immunostaining is a vital tool for improving diagnostic accuracy in Mohs surgery.
It enables reliable differentiation of tumor cells in challenging cases.
The use of specific immunostains contributes to the high efficacy of Mohs micrographic surgery.