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Related Experiment Video

Updated: Jun 3, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

NANOS3 function in human germ cell development.

Vanessa T Angeles Julaton¹, Renee A Reijo Pera

¹Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94305, USA.

Human Molecular Genetics

|March 23, 2011

Summary

The NANOS3 gene is crucial for human germ cell development, impacting germ cell numbers and essential gene expression. This finding offers new insights into infertility and germ cell biology.

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Last Updated: Jun 3, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

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Published on: February 6, 2018

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Area of Science:

Reproductive Biology
Developmental Biology
Genetics

Background:

Human infertility affects many individuals, often due to impaired germ cell development.
Studying human germ cell development is challenging due to limited accessibility, especially during fetal stages.

Purpose of the Study:

To investigate the function of the NANOS gene family in human germ cell differentiation.
To specifically explore the role of NANOS3 in human germ cell development using human embryonic stem cells.

Main Methods:

Examined NANOS-1, -2, and -3 mRNA and protein expression in human gonads.
Reduced NANOS3 expression using morpholinos and short hairpin RNA.
Analyzed effects on germ cell numbers and expression of key germ cell genes.

Main Results:

NANOS3 protein was found in human germ cell nuclei, co-localizing with DNA during mitosis/meiosis.
Reduced NANOS3 expression led to fewer germ cells.
Decreased expression of pluripotency and meiotic genes was observed in NANOS3-deficient germ cells.

Conclusions:

NANOS3 plays a direct, essential role in human germ cell development, including maintenance of pluripotency and meiotic progression.
NANOS3 is a conserved gene critical for germ cell development across species.
Understanding NANOS3 function may advance knowledge of germ cell biology and infertility treatments.