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Updated: Jun 3, 2026

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
Published on: November 27, 2019
Metabolic profiles in children during fasting.
Merel R van Veen1, Peter M van Hasselt, Monique G M de Sain-van der Velden
1Department of Metabolic and Endocrine Diseases, Wilhelmina Children's Hospital Utrecht, University Medical Center, Utrecht, the Netherlands.
New reference values for fasting tolerance tests in children were established. These findings help assess normal glucose homeostasis and identify hypoglycemia risk in pediatric patients.
Area of Science:
- Pediatric Endocrinology
- Metabolic Disorders
- Clinical Chemistry
Background:
- Hypoglycemia is a common childhood metabolic issue.
- Fasting tolerance tests are crucial for diagnosing hypoglycemia.
- Existing reference values for these tests have practical limitations.
Purpose of the Study:
- To establish reference values for key metabolites during fasting in healthy children.
- To provide updated data for accurate interpretation of pediatric fasting tests.
Main Methods:
- Retrospective analysis of 488 fasting tests.
- Exclusion of patients with metabolic/endocrine disorders and abnormal weight.
- Analysis of 167 valid fasting tests from healthy children.
Main Results:
- Hypoglycemia occurred in 31% of tests.
- Three age groups (0-24, 25-84, 85-216 months) were defined based on fasting response.
- Younger children showed faster increases in ketone bodies and decreases in glucose levels compared to older children.
Conclusions:
- Generated new reference values for metabolites during pediatric fasting.
- These values aid in assessing normal fasting responses and identifying hypoglycemia.
- Guidelines for fasting duration and dietary management are crucial for high-risk children.
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