Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
12:03

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

Published on: December 9, 2016

Characteristics of human Ewing/PNET sarcoma models.

Beverly A Teicher1, Rebecca G Bagley, Cecile Rouleau

  • 1Genzyme Corporation, 49 New York Avenue, Framingham, MA 01701, USA. beverlyteicher@aol.com

Annals of Saudi Medicine
|March 23, 2011
PubMed
Summary

Preclinical models, like the A673 Ewing sarcoma cell line, are crucial for studying rare aggressive bone cancers. These models aid in identifying promising therapeutics for Ewing/peripheral neuroepithelioma (PNET) tumors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PRECLINICAL ACTIVITY OF THE B7-H3- TARGETING ANTIBODY-DRUG CONJUGATE (ADC) VOBRAMITAMAB DUOCARMAZINE (VOBRA DUO) IN PEDIATRIC SOLID TUMORS.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

A virtual consultative forum on future reforms to Better Access.

The Australian and New Zealand journal of psychiatry·2026
Same author

Endosialin (CD248) Cancer Role and Therapeutics: 33 Years on.

Molecular cancer therapeutics·2026
Same author

Lamin A/C loss promotes R-loop-mediated genomic instability and poor survival in small-cell lung cancer.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

High-throughput combination screening of Pidnarulex and other G-quadruplex ligands in multi-cell type tumor spheroids.

SLAS discovery : advancing life sciences R & D·2025
Same author

Lamin A/C Deficiency Drives Genomic Instability and Poor Survival in Small-Cell Lung Cancer through Increased R-loop Accumulation.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Oncology
  • Sarcoma Research
  • Translational Medicine

Background:

  • Ewing/peripheral neuroepithelioma (PNET) are aggressive bone sarcomas predominantly affecting young individuals.
  • Developing effective treatments for rare diseases like Ewing sarcoma is challenging due to the need for extensive research and global collaboration.
  • Accurate in vivo models are essential for preclinical testing to identify promising therapeutics with potential clinical impact.

Purpose of the Study:

  • To describe available human Ewing/PNET sarcoma cell lines, focusing on their utility as preclinical models.
  • To highlight the genetic characterization, particularly the EWS/FLI1 fusion transcript, essential for confirming Ewing sarcoma cell lines.
  • To evaluate the A673 Ewing sarcoma cell line as a valuable model for studying disease biology, angiogenesis, and targeted therapies.

More Related Videos

Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues
02:35

Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues

Published on: March 22, 2024

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

Related Experiment Videos

Last Updated: Jun 3, 2026

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
12:03

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

Published on: December 9, 2016

Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues
02:35

Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues

Published on: March 22, 2024

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

Main Methods:

  • Description and genetic analysis of established human Ewing/PNET sarcoma cell lines.
  • Confirmation of cell line identity through detection of the characteristic EWS/FLI1 fusion transcript.
  • In vivo studies using the A673 cell line as xenografts in immunodeficient mice to assess tumor growth, angiogenesis, and response to therapies.
  • Analysis of cell surface protein expression, specifically endosialin, in both cell lines and clinical specimens.

Main Results:

  • Several human Ewing/PNET sarcoma cell lines have been established and genetically validated.
  • The A673 Ewing sarcoma cell line accurately reflects key aspects of the disease, including the EWS/FLI1 translocation.
  • The A673 model supports tumor growth in vivo and has been instrumental in studying Ewing sarcoma angiogenesis and antiangiogenic therapies.
  • Endosialin, a cell surface protein found in clinical Ewing sarcoma, is expressed by the A673 cell line in various disease models.

Conclusions:

  • The A673 Ewing sarcoma cell line serves as a robust preclinical model for investigating Ewing/PNET sarcoma biology.
  • This model is valuable for studying endosialin biology and evaluating endosialin-directed therapies.
  • Availability of validated preclinical models is critical for advancing therapeutic strategies for rare aggressive tumors like Ewing sarcoma.