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Updated: Jun 3, 2026

Real-time Cytotoxicity Assays in Human Whole Blood
Published on: November 7, 2014
The effect of cytostatics and hyperthermia on RAJI human lymphoma cells
Yu P Istomin1, E A Zhavrid, N V Sachivko
1N.N. Alexandrov National Cancer Center of Belarus, Minsk 223040, Belarus. istomin06@mail.ru
Aim:
To evaluate the effect of hyperthermia on cytostatic activity of chemotherapeutic drugs carboplatin, cisplatin, oxaliplatin, carmustine, gemcitabine and etoposide in human lymphoma cell culture.
Methods:
RAJI human lymphoma cells were incubated with cytostatics at 37 °C or 42 °C and evaluated for cell culture growth.
Results:
The number of viable cells after incubation with the drugs (except for gemcitabine) at 42 °C for 30 min was significantly lower than at 37 °C. There were synergism of cytostatic effects of platinum drugs (carboplatin, cisplatin and oxaliplatin) with cytostatic effect of 42 °C and the summation of cytostatic effect of carmustine or etoposide with the action of hyperthermia. The thermal enhancement ratio was 3.0 for oxaliplatin, 2.0 for cisplatin, 1.8-2.4 for carboplatin. The combination of platinum drugs with gemcitabine resulted in a significant enhancement of cytostatic activity.
Conclusion:
Our findings suggest that a gain in sensitivity of RAJI human lymphoma cells to platinum drugs occurs at 42 °C.
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