Albumins and their processing machinery are hijacked for cyclic peptides in sunflower
Joshua S Mylne1, Michelle L Colgrave, Norelle L Daly
1Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia. j.mylne@imb.uq.edu.au
Abstract:
The cyclic peptide sunflower trypsin inhibitor 1 (SFTI-1) blocks trypsin and is a promising drug lead and protein engineering scaffold. We show that SFTI-1 and the newfound SFT-L1 are buried within PawS1 and PawS2, precursors for seed storage protein albumins. Proalbumins are matured by asparaginyl endopeptidase, which we show is required to liberate both ends of SFTI-1 as well as to mature PawS1 albumin. Thus, these peptides emerge from within an albumin precursor by the action of albumin's own processing enzyme.
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