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Updated: Jun 3, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity
Harold J G Meijer1, Hussen Harrun Hassen, Francine Govers
1Laboratory of Phytopathology, Plant Sciences Group, Wageningen University, Wageningen, The Netherlands. Harold.Meijer@wur.nl
Abstract:
In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol.
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