Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
  1. Home
  3. Research Domains

Tumor antigens induced by nontransforming mutants of polyoma virus.

J Silver, B Schaffhausen, T Benjamin

    Cell
    |October 1, 1978

    Related Experiment Videos

    Bone Marrow Sampling and Transplants
    01:22

    Bone Marrow Sampling and Transplants

    846

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    Related Concept Videos

    Bone Marrow Sampling and Transplants01:22

    Bone Marrow Sampling and Transplants

    846
    Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
    The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
    846
  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Tumor Antigens Induced By Nontransforming Mutants Of Polyoma Virus.

    • Polyoma virus tumor antigens were analyzed in various mutants. Hr-t mutants alter minor T antigens, while ts-a mutants produce thermolabile major T antigens, impacting cell transformation.

    Area of Science:

    • Virology
    • Molecular Biology
    • Oncogenesis

    Background:

    • Polyoma virus is a DNA tumor virus that induces tumors in animals.
    • Tumor (T) antigens are viral proteins crucial for oncogenesis.
    • Understanding T antigen structure and function is key to viral oncogenesis.

    Purpose of the Study:

    • To characterize tumor (T) antigens induced by wild-type polyoma virus and its mutants.
    • To investigate the role of T antigens in viral transformation and oncogenesis.
    • To correlate T antigen alterations with specific mutations in polyoma virus.

    Main Methods:

    • Immunoprecipitation using antisera from tumor-bearing animals.
    • Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) for protein separation.
    • Analysis of T antigen species in wild-type and mutant polyoma virus-infected cells.

    Main Results:

    • Wild-type polyoma virus induces a 100,000 dalton major T antigen and four minor T antigens (63K, 56K, 36K, 22K).
    • Hr-t mutants exhibit alterations in minor T antigens; deletion mutants produce very small T antigens (6-9K), while point mutants affect 56K and 22K species.
    • Ts-a mutants produce a thermolabile 100,000 dalton T antigen, crucial for stable transformation.

    Conclusions:

    • Specific mutations in polyoma virus differentially affect T antigen expression and molecular weight.
    • Alterations in minor T antigens are associated with defects in transformation (hr-t mutants).
    • Thermolability of the major T antigen in ts-a mutants correlates with temperature-sensitive transformation.

    Related Experiment Videos

    Bone Marrow Sampling and Transplants
    01:22

    Bone Marrow Sampling and Transplants

    846