Related Experiment Video

Updated: Jun 3, 2026

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
09:19

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

Acute application of cisplatin affects methylation status in neuroblastoma cells

Keiichi Tabata1, Hayato Sakai, Ryosuke Nakajima

  • 1Research Unit of Clinical Medicine, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan. tabata.keiichi@nihon-u.ac.jp

Oncology Reports
|March 23, 2011
PubMed

Insights

Cisplatin alters protein expression and histone methylation in neuroblastoma cells. This study reveals early molecular changes impacting cisplatin's anticancer effects and resistance mechanisms.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Cisplatin is a widely used chemotherapy drug, primarily known for DNA intercalation.
  • The precise effects of cisplatin on cellular protein expression and epigenetic modifications remain incompletely understood.

Purpose of the Study:

  • To investigate the early impact of cisplatin on protein expression in neuroblastoma cells using a proteomic approach.
  • To explore potential alterations in histone methylation following acute cisplatin treatment.

Main Methods:

  • Proteomic analysis using 2-dimensional gel electrophoresis on IMR-32 neuroblastoma cells treated with cisplatin.
  • Assessment of histone methylation status, specifically Histone H3 Lysine 9 (H3K9) methylation.

Main Results:

  • Cisplatin treatment (24 hours) resulted in increased expression of four proteins and decreased expression of one protein.
  • S-adenosyl-L-homocysteine hydrolase, a key enzyme in methylation metabolism, showed the most significant increase in expression.
  • Histone H3K9 methylation levels were reduced after 24 hours of cisplatin exposure.

Conclusions:

  • Acute cisplatin treatment significantly alters protein expression profiles in neuroblastoma cells.
  • Cisplatin exposure impacts cellular methylation status, indicated by changes in S-adenosyl-L-homocysteine hydrolase and histone H3K9 methylation.
  • These findings contribute to a deeper understanding of cisplatin's pharmacological mechanisms, including its anticancer activity, adverse effects, and drug resistance.

More Related Videos

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.