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Expression patterns of imprinted gene Inpp5f-v3 during mouse brain development.
Chen Yan1, He Hongjuan, Xing Yanjiang
1Department of Life Science and Engineering, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, 150001, Heilongjian, China.
Journal of Molecular Histology
|March 23, 2011
Summary
Inpp5f-v3, a gene variant, is specifically expressed in the developing mouse brain. Its expression pattern suggests a role in brain development, particularly in the olfactory bulb and hippocampus.
Area of Science:
- Developmental biology
- Neuroscience
- Genetics
Background:
- Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F).
- It is a paternally expressed imprinted gene located on mouse chromosome 7.
Purpose of the Study:
- To investigate the spatiotemporal expression patterns of the Inpp5f-v3 gene during mouse development.
- To determine the potential role of Inpp5f-v3 in mouse brain development.
Main Methods:
- Northern blotting
- Whole-mount in situ hybridization
- Quantitative real-time PCR (QRT-PCR)
- In situ hybridization
Main Results:
- Inpp5f-v3 transcript (approx. 2.7 kb) was detected exclusively in the brain.
- Expression increased from embryonic day 11.5 to 17.5, peaking at E17.5.
- Strong embryonic expression in cerebral cortex, olfactory bulb, cerebellum, and ventricular zone.
- Postnatal expression localized to the olfactory bulb and hippocampus.
Conclusions:
- Inpp5f-v3 exhibits specific expression in the mouse brain.
- The gene's expression profile suggests a functional role in mouse brain development.
