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Updated: Jun 3, 2026

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
Published on: November 8, 2024
Current role of LC-MS(/MS) in doping control.
Mario Thevis1, Andreas Thomas, Wilhelm Schänzer
1Center for Preventive Doping Research-Institute of Biochemistry, German Sport University Cologne, Cologne, Germany. thevis@dshs-koeln.de
Liquid chromatography-mass spectrometry (LC-MS) enhances sports drug testing by providing sensitive detection of prohibited substances. This advanced technique offers both targeted analysis and broad screening for anti-doping laboratories.
Area of Science:
- Analytical Chemistry
- Sports Science
- Biochemistry
Background:
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is crucial in modern sports drug testing.
- It complements existing immunological and gas chromatography-mass spectrometry (GC-MS/MS) methods.
- Advanced LC-MS/MS offers enhanced capabilities for detecting prohibited substances.
Purpose of the Study:
- To explore the application of LC-MS/MS in sports drug testing.
- To discuss the advantages and limitations of different LC-MS/MS approaches.
- To present recent examples of LC-MS/MS use in anti-doping efforts (2007-2010).
Main Methods:
- Targeted analysis using specific precursor-product ion pairs in low-resolution tandem mass spectrometers.
- Untargeted screening using high-resolution/high-accuracy mass spectrometry to analyze full scan data.
- Measurement and plotting of extracted ion chromatograms for protonated/deprotonated molecules and product ions.
Main Results:
- LC-MS/MS provides fast and sensitive detection of prohibited compounds.
- High-resolution mass spectrometry enables comprehensive screening and confirmation.
- Specific applications demonstrate the utility and challenges of these methods.
Conclusions:
- LC-MS/MS significantly expands the analytical toolkit for anti-doping laboratories.
- Both targeted and untargeted LC-MS/MS approaches offer distinct advantages.
- Careful consideration of method advantages and limitations is essential for effective drug testing.
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