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The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...

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Related Experiment Video

Updated: Jun 3, 2026

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Published on: June 19, 2012

Hybrid-arrested translation.

¹Department of Biochemistry, King's College, University of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 23, 2011

Summary

This summary is machine-generated.

Hybrid arrested translation is a method to identify DNA clones by blocking specific messenger RNA translation. This technique detects gene products by observing their absence in translation patterns, differing from hybrid selection.

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Last Updated: Jun 3, 2026

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

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Published on: June 19, 2012

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Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

Hybrid arrested translation (HAT) is a technique used in molecular biology.
It is used to identify recombinant DNA clones.
This method is similar to hybrid selection or hybrid release.

Purpose of the Study:

To describe the methodology and application of hybrid arrested translation.
To differentiate HAT from hybrid selection/release.
To highlight the utility of HAT in identifying gene products.

Main Methods:

Utilizes the ability of DNA clones to hybridize with specific messenger RNA (mRNA) in a cell-free system.
Prevents the translation of the targeted mRNA.
Relies on the disappearance of a specific gene product from the translation pattern.

Main Results:

Hybrid arrested translation allows for the identification of recombinant DNA clones.
It enables the detection of specific gene products through the absence of their translation.
This method provides a distinct approach compared to hybrid selection, which reveals one or a few polypeptides.

Conclusions:

Hybrid arrested translation is an effective method for identifying gene products.
The technique offers a unique way to study gene expression and function.
HAT complements existing molecular biology techniques for clone identification.