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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 3, 2026

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Enzyme-labeled DNA probes.

J L Woodhead1, R Fallon, H Figueiredo

  • 1Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 23, 2011
PubMed
Summary
This summary is machine-generated.

Molecular biology tools enable rapid DNA probe development for detecting specific genes. These probes accelerate food safety testing and prenatal disease screening, outperforming traditional methods.

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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Last Updated: Jun 3, 2026

Robust 3D DNA FISH Using Directly Labeled Probes
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Molecular biology has developed DNA sequencing tools over the past decade.
  • These tools are increasingly adopted by routine laboratories and clinicians.
  • DNA sequences serve as probes for specific gene detection.

Purpose of the Study:

  • To highlight the applications of DNA probes in various diagnostic and screening contexts.
  • To demonstrate the advantages of DNA probe technology over conventional methods.

Main Methods:

  • Utilizing DNA sequences as probes.
  • Applying these probes for gene detection in different sample types.

Main Results:

  • Rapid screening of food for organisms like Salmonella.
  • Detection of human papillomavirus in cervical smears for neoplasia indication.
  • Antenatal screening for genetic disorders such as sickle cell anemia and β°-thalassemia.

Conclusions:

  • DNA probe technology offers faster and more efficient detection of specific genes and organisms.
  • Applications span food safety, disease diagnostics, and prenatal screening.
  • Molecular biology tools are transforming routine laboratory practices.