Related Experiment Video
Updated: Jun 3, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Luminescent detection of specific DNA sequences
J L Woodhead1, H Figueiredo, A D Malcolm
1Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.
Abstract:
The process whereby part of the energy evolved in a chemical reaction is emitted as light is known as luminescence. When luminol is oxidized by hydrogen peroxide in the presence of horseradish peroxidase (HRP), light is evolved (1). This is shown in Fig. 1. Luciferin not only enhances the reaction, but prolongs the emission over several minutes (2). The light may be measured in a luminometer or visualized as the blackening of photographic film, as will be described. Fig. 1. Oxidation of luminol in the presence of hydrogen peroxide and HRP (the catalyst).
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Sanger Sequencing

