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Related Experiment Video

Updated: Jun 3, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Luminescent detection of specific DNA sequences.

J L Woodhead¹, H Figueiredo, A D Malcolm

¹Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 23, 2011

Summary

Chemiluminescence occurs when luminol is oxidized, producing light. Adding luciferin enhances this reaction, prolonging light emission for several minutes, measurable with a luminometer or photographic film.

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Area of Science:

Biochemistry
Chemiluminescence

Background:

Luminescence is light emission from chemical reactions.
Oxidation of luminol with hydrogen peroxide and horseradish peroxidase (HRP) produces light.

Purpose of the Study:

To describe the chemiluminescent reaction of luminol.
To explain the role of luciferin in enhancing and prolonging light emission.

Main Methods:

Oxidation of luminol using hydrogen peroxide and HRP as a catalyst.
Measurement of light emission using a luminometer or photographic film.

Main Results:

The oxidation reaction produces light.
Luciferin significantly enhances the reaction intensity.
Luciferin prolongs light emission for several minutes.

Conclusions:

The luminol-HRP-hydrogen peroxide system is a source of chemiluminescence.
Luciferin is an effective enhancer and prolonger of luminol-based light emission.