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Related Experiment Video

Updated: Jun 3, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Direct gene transfer into plant protoplasts.

K Lindsey¹, M G Jones, N Fish

¹Department of Biochemistry, Rothamsted Experimental Station, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 23, 2011

Summary

Genetic engineering in plants allows direct gene introduction, bypassing traditional breeding. This is achieved by modifying the Agrobacterium tumefaciens Ti plasmid to carry desired genes into plant cells.

Area of Science:

Plant molecular biology
Genetic engineering
Biotechnology

Background:

Recent advancements in plant molecular biology enable direct gene transfer, overcoming limitations of traditional plant breeding.
The soil bacterium Agrobacterium tumefaciens naturally transfers genetic material via its tumor-inducing (Ti) plasmid.

Purpose of the Study:

To describe the methods for genetically manipulating plants using Agrobacterium tumefaciens.
To highlight the potential of gene transfer for crop improvement and plant science research.

Main Methods:

Exploiting the natural gene transfer mechanism of Agrobacterium tumefaciens.
Isolation and modification of the tumor-inducing (Ti) plasmid.
Insertion of foreign genes and regulatory elements into the Ti plasmid.

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Last Updated: Jun 3, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

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A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

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Published on: January 25, 2018

Scalable Transfection of Maize Mesophyll Protoplasts

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Published on: June 23, 2023

Main Results:

Successful transformation of plants with foreign genes is now possible.
Genetically modified intact plants, cultured tissues, and protoplasts can be produced.
This technique offers a precise method for plant genetic manipulation.

Conclusions:

Agrobacterium-mediated transformation provides a powerful tool for plant genetic engineering.
This technology facilitates the development of improved crop varieties and advanced plant research.
The modification of the Ti plasmid is central to modern plant genetic manipulation strategies.