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Related Experiment Video

Updated: Jun 3, 2026

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

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Nipkow confocal imaging from deep brain tissues.

Yuji Takahara1, Norio Matsuki, Yuji Ikegaya

  • 1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.

Journal of Integrative Neuroscience
|March 23, 2011
PubMed
Summary

Nipkow-disk confocal microscopy can image deep brain tissues up to 150 μm, overcoming light-scattering issues. This technique enables functional calcium imaging in intact neural circuits, advancing neuroscience research.

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Area of Science:

  • Neuroscience
  • Biomedical Imaging
  • Microscopy

Background:

  • Light-scattering in brain tissues limits deep imaging.
  • Multiphoton microscopy is typically used for deep tissue imaging.
  • Confocal microscopy offers high temporal resolution and reduced photobleaching.

Purpose of the Study:

  • To investigate the feasibility of Nipkow-disk spinning-disk confocal microscopy for deep tissue imaging.
  • To demonstrate the capability of this technique for functional neural circuit imaging.

Main Methods:

  • Utilized a Nipkow-disk spinning-disk one-photon confocal microscope.
  • Performed functional multi-cell calcium imaging in intact hippocampal preparations (CA3 neurons).
  • Conducted in vivo imaging of astrocytes in the neocortical layer 1.

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Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
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Last Updated: Jun 3, 2026

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08:26

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Published on: January 13, 2022

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
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Main Results:

  • Successfully imaged brain tissues to a depth of 150 μm.
  • Achieved functional calcium imaging of neurons and astrocytes.
  • Demonstrated high temporal resolution and slow photobleaching during imaging.

Conclusions:

  • Nipkow-disk confocal microscopy is effective for deep brain tissue imaging.
  • This technique expands the utility of confocal microscopy in neuroscience.
  • Enables better understanding of neuronal microcircuitry in vivo.