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[Determination of TTX in biological samples by LC-MS/MS]
Qing Da1, Wei Liu, Bao-hua Shen
1Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. darcy5358@sina.com
Objective:
To propose a method for determination of tetrodotoxin (TTX) in human blood, urine and liver by liquid chromatography with tandem mass spectrometry (LC-MS/MS).
Methods:
Solid phase extraction is used after the samples are precipitated, then the samples will be analyzed by LC-MS/MS.
Results:
The limits of detection were 2 ng/mL in blood and urine and 4 ng/g in liver for TIX respectively, the linear correlation coefficients were not less than 0.9973, both of the intra-day and inter-day precisions were less than 12.80%, the recoveries for all kinds of specimens were more than 47.2%.
Conclusion:
This method is efficient, sensitive and accurate and was successfully validated for implementation in forensic toxicological analysis.
