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Updated: Jun 3, 2026
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
Published on: March 30, 2020
[SNP typing of formalin-fixed tissue in individual identification]
1Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. liny@ssfjd.cn
Objective:
To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome.
Methods:
Genomic DNA was prepared from formalin-fixed tissue. In the event that typing using Sinofiler and MiniFiler kits had failed, 51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). RESULTS Full profiles of X-SNP loci were obtained from the formalin-fixed tissue, while the STR and miniSTR genotyping failed.
Conclusion:
SNP genotyping technique can be used to obtain more information for formalin fixed tissues.
