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Related Experiment Video

Updated: Jun 3, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Integrating surface plasmon resonance biosensor-based interaction kinetic analyses into the lead discovery and

U Helena Danielson1

  • 1Department of Biochemistry and Organic Chemistry, Uppsala University, Sweden. helena.danielson@biorg.uu.se

Future Medicinal Chemistry
|March 24, 2011
PubMed
Summary

Surface plasmon resonance (SPR) biosensor technology is a label-free method crucial for drug discovery. Its enhanced sensitivity and throughput make it ideal for screening fragment libraries and characterizing drug leads.

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Area of Science:

  • Biophysics
  • Drug Discovery
  • Biosensor Technology

Background:

  • Surface plasmon resonance (SPR) biosensor technology is a label-free biophysical technique.
  • It provides kinetic analysis of molecular interactions with information-rich data.
  • Recent advancements enhance its suitability for drug discovery.

Purpose of the Study:

  • To outline the use of SPR biosensor technology in small-molecule drug discovery.
  • To demonstrate how SPR complements other drug discovery techniques.
  • To highlight SPR's value in fragment-based lead discovery.

Main Methods:

  • Utilizing SPR biosensor technology for kinetic analysis of molecular interactions.
  • Employing SPR for screening fragment libraries in drug discovery.

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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Published on: April 17, 2017

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  • Leveraging SPR's kinetic resolution for hit expansion, characterization, and optimization.

    • Main Results:

    • SPR biosensor technology is highly sensitive and offers high throughput for screening.
    • It enables identification of hits based on multiple criteria defined by experimental design.
    • SPR allows extensive characterization of identified leads regarding target and non-target interactions.

    Conclusions:

    • SPR biosensor technology is a valuable tool for drug discovery, particularly for fragment-based strategies.
    • It integrates well with other experimental and computational methods.
    • Widespread adoption of SPR is predicted due to its rich data and capabilities.