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Related Experiment Video

Updated: Jun 3, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Sequence-specific covalent labelling of DNA.

Anna Gottfried¹, Elmar Weinhold

¹Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany.

Biochemical Society Transactions

|March 25, 2011

Summary

This review covers methods for sequence-specific DNA modification, focusing on covalent labeling techniques. These approaches are crucial for advancing biotechnology, diagnostics, and life sciences research.

Area of Science:

Biotechnology
Molecular Biology
Nanotechnology

Background:

Sequence-specific DNA modification is vital for diverse scientific applications.
Covalent labeling ensures stable attachment, preventing label dissociation.
Existing methods include targeting devices and enzyme-directed approaches.

Purpose of the Study:

To review techniques for functionalizing long native DNA.
To highlight methods for sequence-specific DNA modification.
To discuss covalent labeling strategies for DNA.

Main Methods:

Utilizing triple-helix-forming oligodeoxynucleotides for major groove binding.
Employing hairpin polyamides for minor groove binding.
Leveraging enzyme-directed labeling with nicking endonucleases, DNA polymerases, or methyltransferases.

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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

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Main Results:

Demonstrated various targeting devices for DNA functionalization.
Showcased enzyme-directed approaches for DNA modification.
Summarized techniques for covalent labeling of native DNA.

Conclusions:

Multiple methods exist for sequence-specific DNA functionalization.
Covalent labeling offers robust DNA modification solutions.
These techniques are essential for advancing bio- and nano-technology and diagnostics.