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A suite of twelve single nucleotide polymorphism markers for detecting introgression between cutthroat and rainbow
Andrew S Harwood1, Ruth B Phillips
1Salmonid Genetics Laboratory, School of Biological Sciences, Washington State University-Vancouver, 14204 NE Salmon Creek Ave., Vancouver, WA 98686, USA. andrew.harwood@email.wsu.edu
Abstract:
A suite of 12 subspecies and species-specific single nucleotide polymorphism (species-specific SNP) markers was developed to distinguish rainbow trout (RT) Oncorhynchus mykiss from the four major subspecies of cutthroat trout: westslope cutthroat trout (WCT) Oncorhynchus clarki lewisi, Yellowstone cutthroat trout (YCT) Oncorhynchus clarki bouvieri, coastal cutthroat trout (CCT) Oncorhynchus clarki clarki, Lahontan cutthroat trout (LCT) Oncorhynchus clarki henshawi, and their hybrids. Several of the markers were linked to help strengthen hybrid determinations, and sex-specific species-specific SNP assays were also developed.

