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Related Experiment Video

Updated: Jun 3, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

Epstein-Barr virus genetics: talking about the BAC generation.

Regina Feederle¹, Emmalene J Bartlett, Henri-Jacques Delecluse

¹German Cancer Research Centre, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany. h.delecluse@dkfz.de.

|March 25, 2011

Summary

Bacterial artificial chromosome (BAC) technology enables genetic manipulation of herpesviruses (HV), including Epstein-Barr virus (EBV). This facilitates the creation of viral mutants for research and vaccine development.

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Area of Science:

Virology
Molecular Biology
Genetics

Background:

Herpesviruses (HV) are amenable to genetic analysis.
Bacterial artificial chromosome (BAC) technology allows for easy modification of HV genomes in E. coli.
HV BACs can be reintroduced into eukaryotic cells to generate infectious viruses.

Purpose of the Study:

To review the development and application of BAC technology for Epstein-Barr virus (EBV).
To discuss strategies for constructing EBV mutants using BACs.
To highlight the utility of EBV BAC mutants in biological research and medical applications.

Main Methods:

Utilizing homologous recombination techniques in eukaryotic cells.
Cloning and modifying HV genomes on bacterial artificial chromosomes (BACs).
Reintroduction of modified BACs into eukaryotic cells to produce infectious viral mutants.

Main Results:

EBV BAC technology has advanced the study of viral genetic elements.
Mutants derived from EBV BACs have been generated for various research purposes.
These mutants have potential applications in developing preventative vaccines.

Conclusions:

BAC technology is a powerful tool for genetic manipulation of EBV.
Understanding and overcoming technical hurdles in EBV BAC manipulation is crucial.
Available EBV BAC mutants have significantly contributed to virology research.