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Updated: Jun 3, 2026
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
[Scanning of c-kit gene mutations in acute myeloid leukemias using high-resolution melting analysis]
Dong-ming Yao1, Jun Qian, Jiang Lin
1Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China.
Objective:
To detect the common mutations (D816V and N822K) of c-kit gene in acute myeloid leukemia (AML) using high-resolution melting analysis (HRM).
Methods:
HRM analysis was established to screen c-kit mutations in PCR products of c-kit exon 17 in 21 AML patients with t(8;21). PCR products were sequenced to confirm the mutation.
Results:
HRM analysis identified an aberrant melting curve in 6 cases (28.6%), which were confirmed by direct DNA sequencing as one D816V mutation and five N822K mutation.
Conclusion:
HRM analysis is a convenient, rapid, specific and high-throughput technique for scanning c-kit gene mutation in AML.
