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Published on: July 20, 2016
A novel application of the buccal micronucleus cytome assay in oral lichen planus: a pilot study
M Sanchez-Siles1, I Ros-Llor, F Camacho-Alonso
1Oral Medicine Department, Morales Meseguer Hospital, University of Murcia, Spain. marianosasi@um.es
Background:
The most important complication of oral lichen planus is malignancy transformation.
Objective:
The aim of this study was to assess cellular and nuclear morphology in a group of patients with oral lichen planus measured by means of buccal micronucleus cytome assay.
Study Design:
This study included thirty patients with a clinicopathological diagnosis of oral lichen planus (all with atrophic-erosive clinical forms of OLP) and thirty healthy control subjects. Both samples were similar in age and gender. The buccal micronucleus cytome assay protocol consisted of: cell collection from both cheeks with a cytobrush; cell centrifuge; slide preparation, fixation and staining followed by fluorescent microscope analysis. 2 × 10(6) exfoliated cells were screened for nuclear abnormalities: micronuclei, nuclear buds, binucleation, basal and differentiated cells, condensed chromatin, karyorrhectic cells, pyknosis and karyolytic cells.
Results:
Patients with oral lichen planus showed significantly higher frequencies of micronuclei (p<0.001), nuclear buds (p<0.001), binucleated cells (p<0.021) than the control group.
Conclusions:
This method is an easy way for clinicians to assess DNA damage, proliferative potential of basal cells and cell death in buccal cells in cases of oral lichen planus.
